| Comments |
The insert contains BalI, AccI (3), BglII (2), SacI (2), EcoRI (2), and XbaI sites. TaqI RFLP best detected using a 1.1 kb BglII/HindIII segment of the insert, because the 3.8 kb HindIII insert contains a repetitive sequence. Enzyme(s) not detecting polymorphism (in a panel of 5): BamHI, BglII, EcoRI, HindIII, HincII, MspI, PstI, RsaI. Enzymes not detecting polymorphism: PstI, EcoRV, BamHI, BglII, MspI, EcoRI, HindIII, PvuII (10 unrelated Caucasians). Using this probe on TaqI digested human DNA, three RFLP alleles were detected.
A1 (22.00 kb), A2 (18.00 kb), A3 (19.00 kb). Frequency of detection of alleles: A1/A2/A3 = 0.48/0.51/0.01 |
| References |
Chang EH, et al. Human genome contains four genes homologous to transforming genes of Harvey and Kirsten murine sarcoma viruses. Proc. Natl. Acad. Sci. USA 79: 4848-4852, 1982. PubMed: 6289320
Blanche H, et al. A fragment of the human c-Ki-ras1 pseudogene (HMG9 gene symbol KRAS1P), localized to 6p12-p11, detects 3 allele, RFLP. Nucleic Acids Res. 16: 1652, 1988. PubMed: 2894641
Barbara Weiffenbach, personal communication
|
