Restriction digests of the clone give the following sizes (kb): EcoRI--4.2; EcoRI/HindIII--3.1, 1.2; EcoRI/PstI--3.1, 1.2; SmaI--4.1; HindIII--4.0. D3S86 has been mapped to 3p on the human chromosome. Final stringency wash is with 0.1X SSC/0.1% SDS at 50-55C. At higher temperatures, most of the probe is washed off. Enzyme(s) not detecting polymorphism: TaqI, MspI. |
