This host is strongly recommended for screening and amplification of the library.
The host DA1061 (ATCC 47088) suppresses the formation of large non-lambda phage plaques.
Vector
Tissue: embryonic spleen, 78 day
DNA: cDNA
Digest: Complete
Insert size range: 0.3 - 0.5 kb
Coverage: Clones for "complete" coverage: 1000000.0
Media
ATCC® Medium 1592: SM buffer
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Comments
The host DA1061 (ATCC 47088) suppresses the formation of large non-lambda phage plaques. This host is strongly recommended for screening and amplification of the library.
References
Buraczynska MJ, et al. Construction of human embryonic cDNA libraries: HD, PKD1 and BRCA1 are transcribed widely during embryogenesis. Cytogenet. Cell Genet. 71: 197-202, 1995. PubMed: 7656596
