| 產品名稱 | TIME |
|---|---|
| 商品貨號 | B244169 |
| Organism | Homo sapiens, human |
| Tissue | Foreskin; Dermal microvascular endothelium |
| Cell Type | Endothelial cells immortalized with hTERT |
| Product Format | frozen |
| Morphology | Endothelial-like |
| Culture Properties | Adherent |
| Biosafety Level | 2 [Cells contain EMCV viral DNA sequences]
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | Normal |
| Age | Neonatal |
| Gender | Male |
| Applications | The cells represent an effective cell model for studying endothelial cell biology including signal transduction and angiogenesis. |
| Storage Conditions | Liquid nitrogen vapor phase |
| Karyotype | This is a diploid cell line of male origin with a modal chromosome number of 46 and a low rate of polyploidy. The line shows some karyotypic instability at later passages. |
| Images |  |
| Derivation | The telomerase-immortalized human microvascular endothelium cell line, TIME, was derived from a primary culture of neonatal foreskin microvascular endothelial cells (HMVEC) of the dermis. The primary HMVECs were immortalized by infection with the retrovirus WZLblast3:hTERT and cultured in complete growth medium containing blasticidin. |
| Antigen Expression | Positive for integrin alpha v beta 3 Ref  Venetsanakos E, et al. Induction of tubulogenesis in telomerase-immortalized human microvascular endothelial cells by glioblastoma cells. Exp. Cell Res. 273(1):21-33, 2002. PubMed: 11795943 and CD31 (flow cytometry) RefVenetsanakos E, et al. Induction of tubulogenesis in telomerase-immortalized human microvascular endothelial cells by glioblastoma cells. Exp. Cell Res. 273(1):21-33, 2002. PubMed: 11795943
|
| Receptor Expression | The cells express the low density lipoprotein (LDL) receptor and are capable of acetylated LDL uptake. RefVenetsanakos E, et al. Induction of tubulogenesis in telomerase-immortalized human microvascular endothelial cells by glioblastoma cells. Exp. Cell Res. 273(1):21-33, 2002. PubMed: 11795943 |
| Comments | The immortalized cells do not undergo growth arrest in culture due to the exogenous hTERT expression. When plated on Matrigel, TIME cells undergo tubule formation exhibiting capillary-like structures. |
| Complete Growth Medium | The base medium for this cell line is Vascular Cell Basal Medium (ATCC® PCS-100-030), supplemented with Microvascular Endothelial Cell Growth Kit-VEGF (ATCC® PCS-110-041) and 12.5 μg/mL blasticidine. |
| Subculturing | Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Medium renewal: Every 2 to 3 days Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture of Animal Cells: A Manual of Basic Technique by R. Ian Freshney. |
| Cryopreservation | Fetal bovine serum, 90% (v/v); DMSO, 10% (v/v). Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
|
| Culture Conditions | Temperature: 37°C Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| STR Profile | D5S818: 11 D13S317: 9, 11 D7S820: 8, 9 D16S539: 9, 12 vWA: 16, 18 THO1: 6, 7 TPOX: 8 CSF1PO: 11, 12 Amelogenin: XY |
| Population Doubling Level (PDL) | As part of our quality control, we have tested this cell line for its ability to grow for a minimum of 15 population doublings after recovery from cryopreservation. We have also compared its karyotype, telomerase expression level, growth rate, morphology and tissue-specific markers when first recovered from cryopreservation with that of cells at 10+ population doublings to ensure that there is no change in these parameters and that the cells are capable of extended proliferation. |
| Name of Depositor | M McMahon |
| Year of Origin | June 2001 |
| References | Venetsanakos E, et al. Induction of tubulogenesis in telomerase-immortalized human microvascular endothelial cells by glioblastoma cells. Exp. Cell Res. 273(1):21-33, 2002. PubMed: 11795943 Lagunoff M, et al. De novo infection and serial transmission of Kaposi's sarcoma-associated herpesvirus in cultured endothelial cells. J. Virol. 76(5):2440-2448, 2002. PubMed: 11836422 Yi X, et al. Both transcriptional and posttranscriptional mechanisms regulate human telomerase template RNA levels. Mol. Cell. Biol. 19(6): 3989-3997, 1999. PubMed: 10330139 Bodnar AG, et al. Extension of life-span by introduction of telomerase into normal human cells. Science 279: 349-352, 1998. PubMed: 9454332 Caputo JL. Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988 Freshney RI. Culture of Animal Cells: A Manual of Basic Technique, 4th edition. New York: Wiley Liss; 2000. For more information on enzymatic dissociation and subculturing of cell lines see Chapter 10. |
| 梅經理 | 17280875617 | 1438578920 |
| 胡經理 | 13345964880 | 2438244627 |
| 周經理 | 17757487661 | 1296385441 |
| 于經理 | 18067160830 | 2088210172 |
| 沈經理 | 19548299266 | 2662369050 |
| 李經理 | 13626845108 | 972239479 |
