The insert contains the following sites separated by (bp)(approx): SalI- 280- PvuII- 60- PstI- 50- NsiI- 90- SspI- 320- XbaI- 20- NdeI- 420- NsiI- 560- XbaI- 85- SalI. Restriction digests of the clone give the following sizes (kb): SalI--2.65, 2.0; EcoRI--2.65, 1.95; BamHI--4.65; HindIII--4.65; PstI--4.0, 0.59. This clone includes 55 nt of 5'-noncoding sequence with a stop codon in-frame at residue -27, 1245 nt of open reading frame, a stop codon, 581 nt of 3'-noncoding sequence and a poly(A) tail. The probe detects a single band of 2.0 kb in total mRNA from human placenta. |
