| Vector |
Construct size (kb): 3.75 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: UCHL1
Gene name: ubiquitin carboxyl-terminal esterase L1
Contains complete coding sequence?: N
Chromosome: 4; Localization: 4 p14
Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 0.76
Cross references: DNA Seq. Acc.: T07227; EST: EST05116; Primer: M13-21; THC
id: THC100992
DNA Seq. Acc.: X04741; EST: EST05116
Nucleotides 23-279, 275-330 of the insert correspond to
nucleotides 985-729, 734-679 of X04741 with 97% identity. Insert lengths(kb): 0.7599999904632568 Tissue: fetal brain, female, 17-18 wk (Stratagene 936206) Gene product: ubiquitin carboxyl-terminal esterase L1(ubiquitin carboxyl-terminal hydrolase PGP9.5) [UCHL1] |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--2.9, 0.41, 0.35. The insert contains multiple EcoRI fragments. It is important to note these may have been derived from different cDNAs. |
