| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--16.0, 7.6, 6.3, 4.7, 3.45, 2.95, 2.2, 2.0, 1.4, 1.15, 0.52; BglII--13.5, 10.5, 9.4, 7.1, 4.05, 3.5, 2.85; EcoRI--10.0, 9.4, 8.6, 7.2, 6.6, 4.0, 3.5, 2.7; HindIII--17.5, 13.5, 8.6, 6.6, 1.7, 1.25, 0.66; SstI--8.8 (doublet), 7.6, 5.4, 4.7, 3.8, 2.15, 1.9, 1.7, 1.6, 1.45, 1.1, 0.8. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. The insert begins in intron 3' and continues past exon 2 into intron 2. |
| References |
Grosveld G, et al. The chronic myelocytic cell line K562 contains a breakpoint in bcr and produces a chimeric bcr/c-abl transcript. Mol. Cell. Biol. 6: 607-609, 1986. PubMed: 3023859
Heisterkamp N, et al. The first BCR gene intron contains breakpoints in Philadelphia chromosome positive leukemia. Nucleic Acids Res. 16: 10069-10081, 1988. PubMed: 3194194
Chissoe SL, et al. Sequence and analysis of the human ABL gene, the BCR gene, and regions involved in the Philadelphia chromosomal translocation. Genomics 27: 67-82, 1995. PubMed: 7665185
Bruce A Roe, personal communication
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