| Vector Information |
Size (kb): 5.3800001144409180 DESCRIPTION OF VECTOR:
Intact vector size: 5.380
Type of vector: plasmid
Cloning sites: BamHI mLuI BfrI NotI EclXI KspI Asp718 KpnI ApaI SalI XbaI
SacI SphI EcoRV NcoI HindIII
Polylinker sites: BamHI mLuI BfrI NotI EclXI KspI Asp718 KpnI ApaI SalI XbaI
SacI PstI SphI EcoRV NcoI StyI HindIII
Construction: pUC-BM20, proU (pJL32-3), rrnB (pTrc99A), pBR322
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): tetR, ->, 84-1274
replicon: rop (copy number control), 1913-2104
replicon: pMB1, 2532-2532
marker(s): ampR, <-, 3291-4161
transcription terminator: rrnB T2, <-, 4263-4340
transcription terminator: rrnB T1, <-, 4422-4465
MCS: BamHI...HindIII, <-, 4749-4672
coding sequence: proV 5' sequence, <-, 4756-5065
promoter for expression: proU, <-, 5063-5366 Vector: pOSEX2 (plasmid) Promoters: Promoter for expression proU Construction: pUC-BM20, proU (pJL32-3), rrnB (pTrc99A), pBR322 Marker(s):ampR,tetR Construct size (kb): 5.380000114440918 Features: marker(s): ampR
marker(s): tetR
promoter for expression: proU
replicon: pMB1
replicon: rop (copy number control)
MCS: BamHI...HindIII
transcription terminator: rrnB T1
transcription terminator: rrnB T2
coding sequence: proV 5' sequence |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--4.4, 1.05; HindIII--4.6, 0.8; PvuII--5.4. Expression can be induced in cells grown in low osmolarity media by the addition of sodium chloride. Vector does not provide a ribosome binding site or initiation codon. Expression vector allowing osmotically controlled expression of cloned inserts directed by the E. coli proU promoter. This material is being provided with the explicit understanding that it not be used for commercial purposes without prior authorization from the depositor. |
| References |
Herbst B, et al. pOSEX: vectors for osmotically controlled and finely tuned gene expression in Escherichia coli. Gene 151: 137-142, 1994. PubMed: 7828862
Erhard Bremer, personal communication
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