| Insert |
DNA: cDNA Insert lengths(kb): 3.0 Tissue: liver Gene product: phosphofructokinase, liver type( human liver-type 1-phosphofructokinase, Phosphofructokinase, liver type) [PFKL] Alleles: A1, A2 |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--3.0 (doublet); BamHI--3.0, 2.0, 1.0; SalI--6.0; XhoI/PstI--3.0, 1.8, 0.55 (doublet); XbaI--3.0 (doublet). This was constructed from 3 overlapping cDNAs. When transcribed and translated in vitro, a polypeptide of 80 kDa recognized by anti-PFKL antibodies is generated. The clone detects the following genomic restriction fragments (kb), with those not from PFKL indicated by a '*': BamHI--8.6, 7.5, 5.0, 4.2*, 3.9*, 3.3, 2.9, 2.2*; KpnI--14, 11, 4.2, 3.3*, 2.6; HindIII-- >20, 12.3, 5.2. A fragment specific to PFKL can be generated from 3' sequences. The insert contains the complete coding sequences, with 5' and 3' untranslated sequences. The KpnI polymorphic site is located in the 3.3 kb BamHI genomic fragment . |
| References |
Elson A, et al. The structure of the human liver-type phosphofructokinase gene. Genomics 7: 47-56, 1990. PubMed: 2139864
Levanon D, et al. The primary structure of human liver type phosphofructokinase and its comparison with other types of PFK. DNA 8: 733-743, 1989. PubMed: 2533063
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