Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation
derived from existing strain
Isolation date: 1981
Product Format
frozen
Storage Conditions
Frozen: -70°C or colder for 1 week, vapor phase of liquid nitrogen for long-term storage
Type Strain
no
Comments
This is a non-gametocyte-forming strain.
Medium
ATCC® Medium 2196: Malaria medium, complete
Growth Conditions
Temperature: 37°C
Cryopreservation
Only young cells (rings) can be frozen in glycerolyte medium** because their membranes are more robust.
Centrifuge ring stage culture for 5 min at 1800rpm in 50 ml centrifuge tube.
Aspirate supernatant using sterile Pasteur pipet.
Resuspend pellet gently in remaining supernatant.
Slowly add 5 volumes of glycerolyte medium (see below) to 3 volumes pellet dropwise via a syringe as follows:
Add the first volume of glycerolyte and allow the tube to stand for 5 mins. at room temperature.
Add the remaining 4 volumes of glycerolyte and gently agitate.
Aliquot mixture into Nunc screwtop freezing vials and store at -80°C overnight.
Plunge vials into liquid nitrogen (-196°C) the next day and store in liquid nitrogen or liquid nitrogen vapor.
** To formulate glycerolyte medium, combine the following with distilled water to 100 ml: 57.00g glycerol, 1.60g sodium lactate (C3H5NaO3), 30.00mg potassium chloride (KCl), 1.38g sodium dihydrogen phosphate (NaH2PO4). Mix well and adjust pH to 6.8 using concentrated NaOH and/or HCl. Autoclave to sterilize, and store at 4°C.
Name of Depositor
W Trager
Special Collection
ATCC
Year of Origin
1981
References
Bhasin VK, Trager W. Gametocyte-forming and non-gametocyte-forming clones of Plasmodium falciparum. Am. J. Trop. Med. Hyg. 33: 534-537, 1984. PubMed: 6383092
