| Applications |
Codominant segregation of the MspI RFLP was observed in 3 Caucasian families (9 individuals). Enzyme(s) not detecting polymorphism: AluI, ApaI, BamHI, BglI, BglII, Bsp1286, DraI, EcoRI, HaeIII, HincII, HinfI, PstI, PvuII, ScaI, SphI, SstI, StuI, XbaI (tested against 7 unrelated North American Caucasians). |
| Insert |
DNA: genomic Insert lengths(kb): 0.800000011920929 Tissue: fetal liver Gene product: catalase [CAT] Alleles: C1, A1, E1, E2, A2, C2 |
| Comments |
Restriction digests of the clone give the following sizes (kb): Sal--uncut; BamHI/SalI--3.8; SalI/EcoRI--3.8; HindIII/EcoRI--3.0, 0.5, 0.3; AccI/AvaI--3.8; EcoRI/PstI--2.8, 0.9; EcoRI--3.8. Codominant segregation of the MspI RFLP was observed in 3 Caucasian families (9 individuals). The insert contains a portion of intron 1 which separates the exons coding for amino acids 1-21 and 22-79 of the gene. Codominant segregation of the TaqI RFLP was demonstrated in 5 families of 26 individuals. Enzyme(s) not detecting polymorphism: AluI, ApaI, BamHI, BglI, BglII, Bsp1286, DraI, EcoRI, HaeIII, HincII, HinfI, PstI, PvuII, ScaI, SphI, SstI, StuI, XbaI (tested against 7 unrelated North American Caucasians). |
| References |
Wadey RB, Cowell JK. A MspI RFLP associated with the human catalase gene. Nucleic Acids Res. 17: 3332, 1989. PubMed: 2471153
Schroeder WT, et al. Nonrandom loss of maternal chromosome 11 alleles in Wilms tumors. Am. J. Hum. Genet. 40: 413-420, 1987. PubMed: 2883892
Radice P, et al. Loss of heterozygosity in human germinal tumors. Cytogenet. Cell Genet. 52: 72-76, 1989. PubMed: 2612217
Quan F, et al. An RFLP associated with the human catalase gene. Nucleic Acids Res. 13: 8288, 1985. PubMed: 2933636
Quan F, et al. Isolation and characterization of the human catalase gene. Nucleic Acids Res. 14: 5321-5335, 1986. PubMed: 3755525
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