| 產品名稱 | 
      pCMV5Ala5 | 
    
    
      | 商品貨號 | 
      B241562 | 
    
      
      | Designations | 
      pCMV5Ala5 | 
    
      
      | GenBank Number | 
      
        J00136
      | 
    
      
      | Species | 
      Homo sapiens, human | 
    
      
      | Applications | 
      in another host, produces protein coagulation factor IX (plasma thromboplastic component, Christmas disease, hemophilia B)  | 
    
      
      | Vector | 
      Construct size (kb): 7.5 DESCRIPTION OF VECTOR COMPONENT:     Name of vector: pCMV5 Intact vector size: 4.700     Type of vector: phagemid         Vector end: BamHI         Vector end: BamHI      Cloning sites: EcoRI BamHI HindIII   Polylinker sites:         Host range: mammalian cells; Escherichia coli Features (with orientation and position when available):                     promoter: CMV, ->                     terminator: hGH, ->                     replicon: SV40                     replicon: pMB1                     marker(s): ampR, <-                     replicon: f1, <-   Cross references:  | 
    
      
      | Insert | 
      DNA: cDNA DESCRIPTION OF INSERT COMPONENT:              Genome: Homo sapiens         Gene symbol: F9 Genomic copy number: unique           Gene name: coagulation factor IX (plasma thromboplastic component,                      Christmas disease, hemophilia B) Contains complete coding sequence?: U          Chromosome: X; Localization: X q26.3-q27.1         Type of DNA: cDNA          Insert end: BamHI          Insert end: BamHI    Insert size (kb): 2.8    Cross references: DNA Seq. Acc.: J00136 Insert lengths(kb): 2.799999952316284 Gene product: coagulation factor IX (plasma thromboplastic component, Christmas disease, hemophilia B) [F9] Alleles: A1, A2, D2, C1, D1, C2 Target Gene: coagulation factor IX (plasma thromboplastic component, Christmas disease, hemophilia B)  | 
    
      
      | Insert Size (kb) | 
      2.800 | 
    
      
      | Biosafety Level | 
      1 
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.   | 
    
      
      | Shipping Information | 
      Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) | 
    
      
      | Comments | 
      Restriction digests of the clone give the following sizes (kb): BamHI--4.4, 2.9; BglII--7.6; EcoRI--5.0, 2.5; NotI--7.6; SstI--7.1, 0.5. Digestion with EcoRI produces fragments of 5.0 and 2.5 kb. Bacteria may grow slowly due to the high plasmid copy number. Encodes the following mutant form:  K5A.  | 
    
      
      | References | 
      
            Andersson S, et al. Cloning, structure, and expression of a mitochondrial cytochrome P-450 sterol 26-hyroxylase, a bile acid biosnythetic enzyme.  J. Biol. Chem. 264: 8222-8229, 1989. PubMed: 2722778
         
    
        
            Cheung WF, et al. The binding of human factor IX to endothelial cells is mediated by residues 3-11.  J. Biol. Chem. 267: 20529-20531, 1992. PubMed: 1400370
         
    
        
            Cheung WF, et al. The role of the epidermal growth factor-1 and hydrophobic stack domains of human factor IX in binding to endothelial cells.  J. Biol. Chem. 266: 8797-8800, 1991. PubMed: 1851159
         
    
        
            Wing-Fai Cheung, personal communication
          |