| Insert |
DNA: genomic Insert lengths(kb): 40.0 Gene product: DNA Segment, single copy [D16S43] Alleles: B1, B1, B2, B2, B3, B4, A1, A2, B3, B4 |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--19.0, 8.0, 5.5, 4.5, 2.5, 2.4, 2.2, 1.9; BamHI--17.0, 11.0, 7.0, 6.4, 3.1, 1.9; HincII--8.4 (doublet), 6.5, 3.3 (doublet), 3.0, 1.8, 1.75, 1.65, 1.4, 1.3; HindIII--11.0, 6.5, 6.0, 5.4, 4.0 (doublet), 3.5, 2.1, 1.8; BglII--17.0 (doublet), 3.0 (doublet), 2.6. The insert can be excised from the vector with EcoRI, resulting in a 5.5 kb EcoRI vector fragment. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. The PstI and HindIII polymorphisms have a combined PIC value of 0.79 and a combined heterozygosity of 83%. The HindIII polymorphisms have a combined heterozygosity of 33%. Enzyme(s) not detecting polymorphism: BglII, EcoRI, MspI (panel of 5 individuals). |
| References |
Donis-Keller H, et al. A genetic linkage map of the huma genome. Cell 51: 319-337, 1987. PubMed: 3664638
Keith TP, et al. Genetic linkage map of 43 DNA markers on human chromosome 16. Proc. Natl. Acad. Sci. USA 87: 5754-5758, 1990. PubMed: 2377614
Jacobsen SJ, et al. A clinically homogeneous group of families with facioscapulohumeral (Landouzy-Dejerine) muscular dystrophy: linkage analysis of six autosomes. Am. J. Hum. Genet. 47: 376-388, 1990. PubMed: 1975474
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