| Comments |
BamHI--23.0, 17.0, 4.9, 2.3; EcoRI/HindIII--8.0, 4.9, 4.6, 4.1, 3.9, 2.5, 2.3, 2.0, 1.05 + smaller; matching depositors DNA. EcoRI--8.0, 5.9 doublet, 5.1, 2.3, 1.8, 1.5; PstI--12.0, 8.0, 3.6 doublet, 2.2, 2.0, 1.25; HindIII--20.0, 14.0, 9.4, 3.7; IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Isolated by a YN57 or 57 oligonucleotide; RFLPs observed under normal stringency (0.1X SSC). |
| References |
Nakamura Y, et al. New approach for isolation of VNTR markers. Am. J. Hum. Genet. 43: 854-859, 1988. PubMed: 2904220
Nakamura Y, et al. Variable number of tandem repeat (VNTR) markers for human gene mapping. Science 235: 1616-1622, 1987. PubMed: 3029872
Peter J O'Connell, personal communication
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