| Vector |
Construct size (kb): 7.0 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript II KS-
Intact vector size: 2.961
Type of vector: phagemid
Vector end: HindIII
Vector end: SmaI
Cloning sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Polylinker sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->, 3-459
primer site: M13-20, ->
promoter for in vitro transcription: T7, ->
insert detection: lacZ', <-, 816-938
MCS: SacI...KpnI, ->, 657-759
promoter for in vitro transcription: T3, <-
primer site: reverse, <-
replicon: pMB1, 1032-1972
marker(s): ampR, <-, 1975-2832
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: SLC9A1
Other gene symbols: APNH; NHE1
Gene name: solute carrier family 9 (sodium/hydrogen exchanger),
isoform 1 (antiporter, Na+/H+, amiloride sensitive)
Contains complete coding sequence?: U
Chromosome: 1; Localization: 1 p36.1-p35
Tissue: kidney cortex
Type of DNA: cDNA
Insert 5' end: HindIII
Insert 3' end: Modification: blunt ended
Insert size (kb): 4.0
Cross references: DNA Seq. Acc.: M81768 Insert lengths(kb): 4.0 Tissue: kidney cortex Gene product: NA(+)/H(+) antiporter 1( Na+/H+ antiporter, Na+/H+ antiporter protein) [APNH] |
| Comments |
Restriction digests of the clone give the following sizes (kb): HindIII/XbaI--4.2, 3.1; BamHI--3.4, 2.0, 1.5, 0.41; PstI--7.2; SacI--7.2; PvuII--2.7, 2.45, 1.10, 0.56, 0.28. Constructed from a 520 bp HindIII/PstI PCR product and the large PstI/EcoRI fragment from HK-3.5. The SmaI site was lost during cloning, and the vector SacI site was deleted. The insert contains the following restriction sites (approximate kb from the 5' end): BamHI--0.38, 2.3, 3.7; PstI--0.52; SacI--1.1; PvuII--2.1, 2.6, 2.9, 3.9. |
| References |
Takaichi K, et al. Cloning, sequencing, and expression of Na+-H+ antiporter cDNAs from human tissues. Am. J. Physiol. 262: C1069-C1076, 1992. PubMed: 1314485
David G Warnock, personal communication
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