Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format
frozen 1 kit
Storage Conditions
-20°C (or -70°C for long-term storage)
Comments
Corneal Epithelial Cell Growth Kit (ATCC PCS-700-040) contains components that when added to Corneal Epithelial Cell Basal Medium (ATCC PCS-700-030) creates a complete ATCC® Primary Cell Solution&trad; culture environment for epithelial cells derived from normal human corneal. The serum-free medium formulation is designed to support normal corneal epithelial cell morphology as well as promote rapid growth and proliferation. No feeder layers, extracellular matrix proteins or other substrates are required.
The final concentration for each component in ATCC complete corneal epithelial growth medium is as follows:
Apo-Transferrin: 5 µg/mL
Epinephrine: 1.0 µM
Extract P: 0.4%
Hydrocortisone: 100 ng/mL
L-Glutamine: 6 mM
rh Insulin: 5 µg/ml
CE Growth Factor: Proprietary formulation
Complete Growth Medium
Obtain one growth kit from the freezer; make sure that the caps of all components are tight.
Thaw the components of the growth kit just prior to adding them to the basal medium. It is necessary to warm the L-glutamine component in a 37°C water bath and shake to dissolve any precipitates prior to adding to the basal medium.
Obtain one bottle of Corneal Epithelial Cell Basal Medium (485 mL) from cold storage.
Decontaminate the external surfaces of all growth kit component vials and the basal medium bottle by spraying with 70% ethanol.
Using aseptic technique and working in a laminar flow hood or biosafety cabinet, transfer the indicated volume of each growth kit component to the bottle of basal medium using a separate sterile pipette for each.
