Promoters: Promoter for in vitro transcription SP6
Construction: pSP64
Marker(s):ampR
Construct size (kb): 3.0
Features: initiation codon: ATG marker(s): ampR promoter for in vitro transcription: SP6 replicon: pMB1 MCS: EcoRI...NotI restriction site: NcoI signal peptide coding region: NCAM stop codons: 3
Applications
construct to generate mRNA that can be expressed in Xenopus oocytes
vector for constructing cDNA libraries
vector permitting RNA synthesis in vitro
vector with sequences for protein export
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0; NotI--3.0; XhoI--3.0.
Vector designed for in vitro transcription of cloned inserts encoding open reading frames. Transcribed RNA encodes the murine NCAM signal sequence for protein export and will contain the authentic 5'-UTR of X. laevis beta globin on the 5' end.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References
Dommers S, Heinlein UA. Recombinant polypeptides transplanted from pUMA vector-derived cRNAs are translocated through microsomal membranes and exported out of frog oocytes. Biochem. Biophys. Res. Commun. 204: 1346-1351, 1994. PubMed: 7980614
