| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--4.3; EcoRI--4.3, 0.3; HindIII--4.3, 0.2; EcoRI/HindIII--2.7, 2.0, 0.3; PstI--3.2, 1.5; consistent with deposited DNA. Codominant segregation of the MspI RFLP was observed in 43 three-generation families. Linkage has been established between D15S45 and Marfan syndrome in 17 families (theta = 0.02, LOD score = 7.318). Enzyme(s) not detecting polymorphism: MspI, TaqI, RsaI, PstI, BglII, PvuII. |
| References |
Fujimoto E, et al. Isolation and mapping of a polymorphic DNA sequence (pEFZ33) on chromosome 15 [D15S45]. Nucleic Acids Res. 16: 10946, 1988. PubMed: 2905042
Kainulainen K, et al. Marfan syndrome: no evidence for heterogeneity in different populations, and more precise mapping of the gene. Am. J. Hum. Genet. 49: 662-667, 1991. PubMed: 1882844
Yusuke Nakamura, personal communication
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