| Comments |
Restriction digests of the clone give the following sizes (kb in order from the CEN end): NruI--100, 130, 40, 410; MluI--230, 105, 100, 245. A complete cDNA probe detects a 70 kb BssHII fragment in both YAC and human DNA. The gene is located near the center of the insert, with the 5' end toward the yeast centromere. It is contained in a 100 kb MluI fragment. When transferred to mouse L-cell line A-9 (GM00346B, HPRT-negative), the YAC conferred the ability to grow in HAT medium. |
| References |
Gnirke A, Huxley C. Transfer of the human HPRT and GART genes from yeast to mammalian cells by microinjection of YAC DNA. Somatic Cell Mol. Genet. 17: 573-580, 1991. PubMed: 1767336
Imai T, Olson MV. Second-generation approach to the construction of yeast artificial-chromosome libraries. Genomics 8: 297-303, 1990. PubMed: 2249850
Huxley C, et al. The human HPRT gene on a yeast artificial chromosome is functional when transferred to mouse cells by cell fusion. Genomics 9: 742-750, 1991. PubMed: 2037299
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