Insert contains the following sites separated by (bp)(approx): EcoRI/PstI- 50- HaeII- 100- HaeII- 15- HincII- 70- XhoII- 15- FokI/PstI- 265- XhoII- 35- HinfI- 400- HinfI/PvuII- 310- EcoRI. Restriction digests of the clone give the following sizes (kb): EcoRI--3.6, 1.2; HincII--4.2, 0.26; PvuII--2.7, 1.3, 0.36; SphI--3.8, 0.41; HindIII--4.5. This is a shorter version of Myf-5 which extends from the PstI site at nucleotide 185 to 1430. It does not include the complete coding sequence, which extends from nucleotide 43 through 807. Shows weak cross-hybridization to the mouse MyoD1 probe. Amino acid sequence has a region highly similar to myc proteins and the Drosophila developmental factors achaete scute and twist. The 3' XhoII/EcoRI fragment has been used as a specific probe. A 750 bp PstI/PvuII fragment, which does not contain the myc homology region of Myf5, was used to identify murine Myf-5 and herculin clones from a genomic library. |
