| 產品名稱 |
SW 1353 [SW 1353, SW-1353] |
| 商品貨號 |
B165778 |
| Organism |
Homo sapiens, human |
| Tissue |
bone |
| Product Format |
frozen |
| Morphology |
fibroblast |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
chondrosarcoma |
| Age |
72 years |
| Gender |
female |
| Ethnicity |
Caucasian |
| Applications |
transfection host |
| Storage Conditions |
liquid nitrogen vapor temperature |
| Karyotype |
hyperdiploid 47, XX, +7; Except for the trisomic N7 no other chromosome markers are evident |
| Derivation |
The SW 1353 cell line was initiated by A. Leibovitz at the Scott and White Clinic, Temple, Texas in 1977 from a primary grade II chondrosarcoma of the right humerus obtained from a 72 year old female Caucasian. |
| Clinical Data |
72 years Caucasian female The SW 1353 cell line was initiated by A. Leibovitz at the Scott and White Clinic, Temple, Texas in 1977 from a primary grade II chondrosarcoma of the right humerus obtained from a 72 year old female Caucasian. |
| Antigen Expression |
Antigen expression: Blood type O- |
| Comments |
The initial culture medium was L-15 containing cortisone and insulin plus 10% fetal bovine serum and antibiotics. A frozen ampule at passage 12 was received at the ATCC in January, 1982. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. (Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation)
|
| Subculturing |
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended
Medium Renewal: 2 to 3 times per week |
| Cryopreservation |
Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor temperature |
| Culture Conditions |
Temperature: 37°C Atmosphere: air, 100% |
| STR Profile |
Amelogenin: X CSF1PO: 12 D13S317: 12,13 D16S539: 11,12 D5S818: 10,11 D7S820: 9,11 THO1: 6,9 TPOX: 8,11 vWA: 16,17 |
| Isoenzymes |
AK-1, 1 ES-D, 2 G6PD, B GLO-I, 2 PGM1, 1 PGM3, 2 |
| Name of Depositor |
A Leibovitz |
| Deposited As |
Homo sapiens |
| Passage History |
A frozen ampule at passage 12 was received at the ATCC in January, 1982. |
