| 產品名稱 | D-17 |
|---|---|
| 商品貨號 | B164314 |
| Organism | Canis familiaris, dog |
| Tissue | bone; derived from metastatic site: lung |
| Product Format | frozen |
| Morphology | epithelial |
| Culture Properties | adherent |
| Biosafety Level | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | osteosarcoma |
| Age | 11 years |
| Gender | female |
| Strain | poodle |
| Applications | This cell line may be used as a transfection host. |
| Storage Conditions | liquid nitrogen vapor phase |
| Karyotype | modal number = 63; range = 58 to 70. There were 25 to 30 biarmed chromosomes not common to the diploid dog karyotype present in all cells examined. Note: Cytogenetic information is based on initial seed stock at ATCC. Cytogenetic instability has been reported in the literature for some cell lines |
| Images |  |
| Derivation | Derived from an osteosarcoma metastatic to the lung in an 11-year-old female poodle. |
| Tumorigenic | Yes |
| Effects | Yes, in immunosuppressed mice Yes, in nude mice |
| Virus Susceptibility | Canine adenovirus 1
Canid herpesvirus 1 , Canine herpesvirus Canine parainfluenza virus |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week |
| Cryopreservation | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| Name of Depositor | WA Nelson-Rees |
| Deposited As | Canis familiaris |
| Year of Origin | 1969 |
| References | Temin HM, Watanabe S. Helper cell. US Patent 4,650,764 dated Mar 17 1987 Riggs JL, et al. Immunofluorescent studies of RD-114 virus replication in cell culture. J. Gen. Virol. 25: 21-29, 1974. PubMed: 4372315 |
| 梅經理 | 17280875617 | 1438578920 |
| 胡經理 | 13345964880 | 2438244627 |
| 周經理 | 17757487661 | 1296385441 |
| 于經理 | 18067160830 | 2088210172 |
| 沈經理 | 19548299266 | 2662369050 |
| 李經理 | 13626845108 | 972239479 |
