| 產品名稱 | 127TAg |
|---|---|
| 商品貨號 | B162656 |
| Organism | Mus musculus, mouse |
| Tissue | embryo |
| Cell Type | fibroblast immortalized with SV40 large T antigenSV40 large T antigen transfected |
| Product Format | frozen |
| Morphology | fibroblast |
| Culture Properties | adherent |
| Biosafety Level | 2 cells containing SV40 viral DNA sequences
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age | 14.5 days gestation embryo |
| Applications | DNA repair studies |
| Storage Conditions | liquid nitrogen vapor phase |
| Images |  |
| Derivation | 127TAg (Pms2 null [-/-]) is a mouse embryonic fibroblast (MEF) cell line derived from Pms-2 null embryos at day 14.5 of gestation. The Pms-2 deficient mice were constructed using ES-D3 [D3] embryonic stem cells [PubMed: 9096356]. The cell line was established by transfection with an expression vector for SV40 large T antigen [PubMed: 8538772]. The cells are transgenic for lambda LIZ (Lac I/cII). |
| Comments | 127TAg (Pms2 null [-/-]) is a mouse embryonic fibroblast (MEF) cell line derived from Pms-2 null embryos at day 14.5 of gestation. The Pms-2 deficient mice were constructed using ES-D3 [D3] embryonic stem cells [PubMed: 9096356]. The cell line was established by transfection with an expression vector for SV40 large T antigen [PubMed: 8538772]. The cells are transgenic for lambda LIZ (Lac I/cII). |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing | Protocol:
Interval: Maintain cultures at a cell concentration between 3 X 10(3) and 1 X 10(4) cells/cm2. Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:8 is recommended Medium Renewal: Every two to three days |
| Cryopreservation | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
| Population Doubling Time | 17 hours |
| Name of Depositor | RW Sobol |
| Year of Origin | 2000 |
| References | Sobol RW, et al. Requirement of mammalian DNA polymerase-beta in base-excision repair. Nature 379: 183-186, 1996. PubMed: 8538772 Sobol RW, et al. Base excision repair intermediates induce p53-independent cytotoxic and genotoxic responses. J. Biol. Chem. 278: 39951-39959, 2003. PubMed: 12882965 Narayanan L, et al. Elevated levels of mutation in multiple tissues of mice deficient in the DNA mismatch repair gene Pms2. Proc. Natl. Acad. Sci. USA 94: 122-127, 1997. PubMed: 9096356 Sobol RW, et al. Mutations associated with base excision repair deficiency and methylation-induced genotoxic stress. Proc. Natl. Acad. Sci. USA 99: 6860-6865, 2002. PubMed: 11983862 Sobol RW, et al. The lyase activity of the DNA repair protein beta-polymerase protects from DNA-damage-induced cytotoxicity. Nature 405: 807-810, 2000. PubMed: 10866204 |
| 梅經理 | 17280875617 | 1438578920 |
| 胡經理 | 13345964880 | 2438244627 |
| 周經理 | 17757487661 | 1296385441 |
| 于經理 | 18067160830 | 2088210172 |
| 沈經理 | 19548299266 | 2662369050 |
| 李經理 | 13626845108 | 972239479 |
