| Comments |
The insert contains HincII sites at 0.9 and 2.0 kb from the naturally occurring EcoRI site. Restriction digests of the clone give the following sizes (kb): EcoRI--3.7, 2.4; HincII--2.5, 1.5, 1.1, 0.95; PstI--6.1; HindIII--5.3, 0.4 (doublet); BglI--uncut. Detects a major genomic EcoRI fragment of 3.0 kb corresponding to the pseudogene and a faint genomic EcoRI fragment of 3.8 kb from FUCA1. Enzymes not detecting polymorphism: AvaII, BamHI, BglI, BglII, EcoRI, EcoRV, HincII, HindIII, KpnI, MboI, MspI, PstI, PvuII, RsaI, SacI, SstI, StuI, TaqI, XbaI, XmnI (N=9). |
| References |
Daly ME, Perry DJ. BclI RFLP in fucosidase pseudogene (FUCA1P). Nucleic Acids Res. 18: 5583, 1990. PubMed: 1977129
Carritt B, Welch HM. An alpha-fucosidase pseudogene on human chromosome 2. Hum. Genet. 75: 248-250, 1987. PubMed: 3030924
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