Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format
freeze-dried
Storage Conditions
Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C Live Culture: See Propagation Section
Preceptrol®
no
Genotype
lacIq+ delta(lacZ)M15+ tetA+ tetR+
Genome Sequenced Strain
Pseudomonas aeruginosa PAO1, complete genome
Type Strain
no
Comments
For use with the expression vector pUCP19 (ATCC 87110).
Phenotype verified: tetR.
Constructed by stable integration of a mini-D3112 transposable element containing sequences allowing lacZalpha complementation.
Purified genomic DNA from this organism is available as ATCC 47085D-5.
Genome sequenced strain
Medium
ATCC® Medium 1631: LB medium (ATCC medium 1065) with 10 mcg/ml tetracycline
Growth Conditions
Temperature: 30°C
Atmosphere: Aerobic
Name of Depositor
RR Karkhoff-Schweizer
Special Collection
NCRR Contract
References
Karkhoff-Schweizer RR, Schweizer HP. Utilization of a mini-Dlac transposable element to create an alpha-complementation and regulated expression system for cloning in Pseudomonas aeruginosa. Gene 140: 7-15, 1994. PubMed: 8125342
Stover CK, et al. Complete genome sequence of Pseudomonas aeruginosa PA01, an opportunistic pathogen. Nature 406: 959-964, 2000. PubMed: 10984043
